pgex6p1 ube2s ubd Search Results


ubiquitin conjugating e2 enzyme ube2s  (Addgene inc)
90
Addgene inc ubiquitin conjugating e2 enzyme ube2s
Assembly of Rub1-Rub1 homodimers monitored by Coomassie stained SDS-PAGE. A, Formation of Rub1K4F,T72R-Rub1K4F,T72R homodimer catalyzed by ubiquitin E1 and UBE2K (as E2). B, Formation of Rub1T72R-Rub1T72R homodimers catalyzed by ubiquitin E1 and <t>UBE2S</t> (as E2). The products are shown in two lanes: one collected after the reaction and the other after centrifugation to remove precipitated E1 and E2
Ubiquitin Conjugating E2 Enzyme Ube2s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgex6p1+ube2s+ubd/pGEX6P1-UBE2S-UBD+(Plasmid+%2366713)/pmc07236025-52-16-21
Average 90 stars, based on 1 article reviews
ubiquitin conjugating e2 enzyme ube2s - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


Assembly of Rub1-Rub1 homodimers monitored by Coomassie stained SDS-PAGE. A, Formation of Rub1K4F,T72R-Rub1K4F,T72R homodimer catalyzed by ubiquitin E1 and UBE2K (as E2). B, Formation of Rub1T72R-Rub1T72R homodimers catalyzed by ubiquitin E1 and UBE2S (as E2). The products are shown in two lanes: one collected after the reaction and the other after centrifugation to remove precipitated E1 and E2

Journal: Journal of mass spectrometry : JMS

Article Title: Top-down analysis of novel synthetic branched proteins

doi: 10.1002/jms.4303

Figure Lengend Snippet: Assembly of Rub1-Rub1 homodimers monitored by Coomassie stained SDS-PAGE. A, Formation of Rub1K4F,T72R-Rub1K4F,T72R homodimer catalyzed by ubiquitin E1 and UBE2K (as E2). B, Formation of Rub1T72R-Rub1T72R homodimers catalyzed by ubiquitin E1 and UBE2S (as E2). The products are shown in two lanes: one collected after the reaction and the other after centrifugation to remove precipitated E1 and E2

Article Snippet: Enzymatic assembly of Rub1 T72R -Rub1 T72R homodimer Rub1 T72R (10 mg) was incubated with 20-μM ubiquitin-conjugating E2 enzyme UBE2S (UBE2S-UBD, Addgene #66713), 500-nM UBE1, 10 mM creatine phosphate, 5 mM MgCl 2 , 5 mM ATP, and creatine phosphokinase in 50-mM Tris-HCl buffer (pH 8) for approximately 16 hours.

Techniques: Staining, SDS Page, Ubiquitin Proteomics, Centrifugation